- Directors: Len Wiseman
- Producers: Robert Bernacchi, Gary Lucchesi, Tom Rosenberg
- Writers: Story, Kevin Grevioux, Len Wiseman, Danny McBride, Screenplay, Danny McBride
- Genres: Action, Fantasy, Horror, Thriller
- Actors: Kate Beckinsale, Scott Speedman, Michael Sheen, Bill Nighy, Shane Brolly
Perched on the ledge of a building in a rainy night, two black-garbed vampires known as Death Dealers track a pair of werewolves who are walking on the street below in their human form. The vampires, Selene and Rigel, specialize in assassinating an ancient species of werewolves known as Lycans. Selene’s motivation goes beyond duty; she also wants revenge, for she believes that Lycans slaughtered her family when she was a child. The vampires believe that they defeated the werewolves many centuries ago and killed their leader, Lucian, and that they must now kill off the survivors. As the vampires follow the Lycans into a subway station, the werewolves open fire with submachine guns. After the Lycans retreat from Selene’s barrage of machine pistol fire, she tracks them to their lair, where she hears loud howling and finds out that the Lycans have developed a new high-tech bullet to kill the vampires.
When Selene arrives at the vampire coven’s ornate, gated mansion, she recounts the evening’s events, and urges an attack on the Lycans, but the vampire regent Kraven tells her to drop the matter. Selene realizes that the Lycans may have been following a human, Michael Corvin, which would be very unusual behavior and secretly continues her investigation. Meanwhile, in an underground Lycan lair, a scientist named Singe is testing blood from kidnapped descendants of the ancient Corvinus family, to try to find a pure source of the ancient and powerful Corvinus blood type. Soon after Selene finds Michael, the pair are attacked by werewolves, including Lucian, the original Lycan leader, who still lives. After Lucian bites Michael on the shoulder, Selene helps Michael escape, and the two become romantically attracted to each other.
Selene and Michael flee the Lycan lair, now an enemy from both Lycan and Vampire covens. Back at the mansion, Singe’s blood seeps through the trapdoor of the sarcophagus of the remaining Elder, Markus, a carrier of the original Corvinus Strain. When his eyes open, they are vampire blue but then turn pitch black, the eyes of a hybrid…