- Directors: Len Wiseman
- Producers: Len Wiseman, Danny McBride, Kevin Grevioux
- Writers: Characters, Len Wiseman, Danny McBride, Kevin Grevioux, Story, Len Wiseman, Danny McBride, Screenplay, Danny McBride
- Genres: Action, Fantasy, Horror, Thriller
- Actors: Kate Beckinsale, Scott Speedman, Tony Curran
The movie opens in the year 1202 AD with an army led by three vampire elders (Markus, Viktor, and Amelia) arriving at a Werewolf ravaged village. After killing several werewolves, Viktor and Amelia capture their target: Markus’s brother William Corvinus, the first and most powerful werewolf. Viktor orders that William be imprisoned in a secret location forever.
The story then continues the present-day timeline, after the events of the first film. Selene takes Michael to a safe house so that she can return to the mansion to confront Kraven. She knows Kraven intends to kill Markus and plans to stop him. However, Singe’s blood has already awakened Markus, turning him into a hybrid, who then wipes out Kraven and his men, and destroys the mansion. He learns Selene and Michael’s location from camera surveillance of various safe houses and leaves to track them down. Markus confronts Selene and Michael, who evade him and hide in a warehouse.
Lorenz Macaro, an elderly and imposing man, sends in a team of “Cleaners” to investigate the aftermath from the final battle in the first movie. When Macaro examines Viktor’s body he finds a metal disk attached to his ribcage which is the match to Sonja’s pendant, which is in the possession of Selene and Michael. Now knowing that the pendant is of some importance to Markus, Michael and Selene set out to solve its mystery. Selene recalls that she’d seen it as a child, but doesn’t know its significance. To find answers, they travel to the hideout of the exiled vampire historian, Andreas Tanis.
Selene leads the cleaners to the prison to confront and destroy Markus, but he has already freed William. A battle ensues in which William bites the Cleaners and begins turning them into Lycans. Michael, presumed to be dead inside his bodybag, suddenly regenerates and joins the fight in his Hybrid form. Michael ultimately kills William, while Selene kills Markus by pushing him into the spinning blades of a crashed helicopter. It is then revealed that Selene can now stand in the sun. As the film ends, Selene narrates that she fears the days ahead, as the death of the Elders will cause nothing but chaos; but she is nevertheless hopeful.